Raw OD₆₀₀ values are non-linear at higher densities due to secondary light scattering. We apply a calibrated 4th-order polynomial to determine true cell density. Doubling time (g) is derived from the growth rate (μ) using non-linear regression.

Worksheet Parameters

Incubation Window

Define your Inoculation and Harvest times. Use the +1D toggle if the culture grows overnight. The tool calculates total duration to determine the required starting density.

Target Specifications

Set your desired Harvest OD₆₀₀ and Final Volume. For standard mitochondrial preps in S. cerevisiae, log-phase harvest is typically targeted between OD 0.5 – 0.8.

Source Culture (Seed & Dil.)

To maintain accuracy above OD 1.0, you must measure a diluted sample:

Seed OD: The raw value displayed on your spectrophotometer for the diluted aliquot (e.g., 0.45).
Dil. Factor: The multiplier used for that aliquot. If you added 100µl cells to 900µl media, enter 10.

Logic: True Density = [Polynomial Corrected Seed OD] × [Dilution Factor].

Growth Rate (DT)

The time required for the population to double. Default for wild-type yeast in YPD at 30°C is 1.5h. Adjust this value for different media (e.g., Synthetic Defined), temperatures, or petite mutants.

OSMANLAB

Faculty of Biology
Ludwig-Maximilians-University Munich

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We would like to express our gratitude to Carolin Bleese (Faculty of Biology, LMU) for providing the team photography.

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