A 12-hour time-lapse capturing the transition from heteroplasmy to homoplasmy in S. cerevisiae. A heteroplasmic zygote was generated containing two mtDNA variants: one encoding Atp6-mKate2 (visualized in magenta) and another encoding Atp6-mNeonGreen (visualized in cyan). Imaged within a CellASIC ONIX2 microfluidic chamber, the movie illustrates the segregation of mitochondrial genomes during successive budding events. While initial cells exhibit signals from both variants (heteroplasmy), the lineage progressively drifts toward single-color fluorescence, demonstrating the spatial sequestering and inheritance patterns of mtDNA-encoded proteins.