This five-hour timelapse captures the elegant choreography of mitochondrial inheritance in budding yeast (S. cerevisiae). Captured on a Nikon Ti2 microscope using a 100x objective, the movie shows a merge of widefield and fluorescent channels. Mitochondria are visualized via a mitochondrially targeted mKate2 fluorophore, revealing a continuous, branching network that undergoes constant fission and fusion.
To capture the mitochondrial network throughout the cell cycle, 41 z-planes were acquired at each timepoint and processed into the z-projections shown here.
This work received a Honorable Mention in the 2022 Nikon Small World in Motion Competition.