Live-cell microscopy of dividing yeast cells (S. cerevisiae). Mitochondria are visualized with a mitochondrial-targeted red fluorescent protein (mKate2). Mitochondrial DNA is labeled with the mitochondrial LacO-LacI system. LacO repeats are integrated upstream of the COX2 gene. A fusion protein consitisint of the lac-repressor (LacI) and the green fluorescent protein mNeonGreen is expressed from a gene integrated into the nuclear genome and targeted to mitochondria. The movie spans two hours. Images were taken every 15 minutes. z-projections are shown. The images were acquired with a Nikon Ti2 widefield microscope equipped with a Photometrix 95B camera.