A 5-hour time-lapse capturing the coordinated segregation of the endoplasmic reticulum (ER) and the nuclear genome during Saccharomyces cerevisiae cell division. The ER architecture is visualized using the translocon component Sec63 tagged with mKate2/mCherry (magenta), highlighting the nuclear envelope and peripheral ER distribution. Simultaneously, a specific genomic locus is tracked using 112 LacO repeats integrated at the URA3 locus and labeled via a cyan fluorescent reporter. The movie transitions from a composite overlay of brightfield and fluorescence channels to a high-contrast dual-channel fluorescence view, detailing the precise timing of nuclear migration and ER inheritance into the developing daughter cell.